FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87372739
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1513070.17317415218034998No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1283260.14687189788109997No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1127150.1290047688673237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT543750.015.2032623
GTCACTC522600.014.99457558
GGTCACT522700.014.9058457
GGAGGTC560550.014.42211154
GAGGTCA566100.014.3797995
AGCTTAG423550.014.15539345
AACTCTA202500.014.05383845
TCAGACG207650.013.874182
GGCATAG430200.013.85317835
AGGTCAC591750.013.6004796
ACTCTCC587550.013.47647111
AGGGATC320900.013.2492572
ATAGGGG460350.013.23913938
TGGCATA472900.012.70736834
GTTGTCG53900.012.64699326
GAGCTTA483900.012.53435844
TGCCCGT339900.012.4303244
TATATAC93800.012.0203457
TGGGAGG536550.011.9386152
TGCGTCG73050.011.85895710
Produced by FastQC (version 0.11.5)