FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47914821
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1081910.22579861041325816No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC793280.165560464057666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG49100.020.355555
ACCGCGT50900.019.7560256
ACGCTAC135500.018.84384524
TCGGCGC258150.018.83982345
AACGCTA134850.018.83444623
TTCGGCG262600.018.54724344
TTCGACG24100.018.3890386
GTTAGTT294000.018.34158744
CGCTACC139950.018.1803725
AGACCGC56300.017.7123724
TTCGCGC16550.017.534837
CCGCGTT58050.017.283937
CGCGTTC59900.016.8252328
AAAACGC155000.016.313421
CGTTCGA46350.015.96843444
TCCGTAC318100.015.9012483
TCACGCT172250.015.6983898
CGTACGC325000.015.4593865
CCGTACG325100.015.4546294
TCGACGA28150.015.4237067
Produced by FastQC (version 0.11.5)