FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56392497
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4362900.7736667521567631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG151850.015.42550745
ATAGGGG164900.014.28360638
GGCATAG166150.014.17944335
CATAGGG183550.012.94243237
GTTGTCG29850.012.51207126
GAGCTTA193650.012.33970344
GCATAGG192800.012.25227336
GTCACTC265000.012.0481798
GGGAGGT294250.011.8366423
GGTCACT267700.011.73378857
TGGCATA212550.011.221312534
ACTCTCC286700.011.19850811
GGAGGTC295250.011.1642194
AGGTCAC303050.010.8105346
TAGGGGA306950.010.15775539
GAGGTCA330750.010.1567455
CACTCTC336550.09.54666810
CACCGAA276300.09.51186145
TCACTCT354850.09.3261729
TCTATAG293250.09.2069813
Produced by FastQC (version 0.11.5)