FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45201554
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG464430.10274646752189096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26550.025.338636
ACCGCGT89500.023.6812346
GACCGCG92450.023.0229365
AGACCGC95700.022.1705384
CGGTATA43650.020.66974820
CCGCGTT102650.020.6036427
TCGACGA33650.020.3265447
CAGACCG110800.019.3724773
CGCGTTC111400.019.0257058
CGACGAG37850.018.011588
GAACGAT123000.016.90274645
AATACGG125400.016.6688932
TCACGCT168700.016.3912418
ATACGGA127800.016.3558633
TCAGACC138050.016.0049362
AACGCTA164700.015.96967123
ACGCTAC165600.015.80135924
CGCTACC173950.015.12046425
ACGCTCA194600.014.82245810
GTTCGAC45150.014.800465
Produced by FastQC (version 0.11.5)