FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50192411
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT952340.18973784702233173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22650.025.8276566
AGACCGC186400.025.7831024
GACCGCG193100.024.923465
CAGACCG198600.024.3918443
ACCGCGT197450.024.3060046
CGCGTTC206750.023.2561598
CCGCGTT212400.022.59527
TCAGACC233300.021.1883372
GAACGAT229050.019.78396445
TCGACGA30950.019.410227
AATACGG251250.018.17000232
ATACGGA247800.018.15965833
TACGGAG249300.017.942234
TGCGCGT57200.017.93713245
CTCAGAC324150.017.899271
GCGTTCT271750.017.7928649
AGAACGA265650.017.2784444
TCGGCGC108350.016.75825545
ATGCGCG61550.016.7425544
CGACGAG34550.016.7364778
Produced by FastQC (version 0.11.5)