FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50056705
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1883190.3762113387207568No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT698380.1395177728937612No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG605780.12101875263263932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG52650.033.073846
TCGACGA61000.028.8046937
CGGTATA85150.028.08604820
GTTCGAC62150.026.9323255
CGACGAG61600.026.8806118
GACCGCG147750.026.2361355
GCCGGCT378200.025.9052371
AGACCGC151200.025.7118914
ACCGCGT151300.025.54626
CAGACCG162250.024.1965143
CCGCGTT166400.023.2144837
CCGGCTT466750.023.1950132
TCACGCT496550.021.8930368
CGCGTTC177600.021.788518
AGTTCGA79950.021.6959154
TTCACGC502200.021.633297
ATGACGG103700.021.3480616
AACGCTA504950.020.9674523
ACGCTAC505850.020.9127524
CGCTACC515500.020.42584625
Produced by FastQC (version 0.11.5)