FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45922359
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG966540.21047263708730643No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA605960.13195315162272042No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA492720.10729413965863557No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG492500.10724623271204338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT218550.021.9815031
CCGGCTT262800.020.3504752
CTCGGTT122550.020.0139081
GCGGACT63650.019.1589472
TCACGCT266850.018.7431688
TTCACGC268100.018.6473877
GTCCGTT35400.018.0689281
GGCGGAC77900.018.0122471
CGGCTTC325400.017.3758353
CCCCCCT29400.017.1600281
CCCGTAT32050.017.1466431
CACGCTC292150.017.066119
CCCGGAT81900.016.940031
CGGTTTT130900.016.9132163
CTCCGAT48700.016.880341
CCCGGCT68950.016.7898251
ACGCTCA300750.016.61550710
TCGGTTT139700.016.3631922
CGTGCGT75750.015.97976535
CGGACTT83300.015.9359423
Produced by FastQC (version 0.11.5)