FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50618978
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG699080.13810630471440968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187650.018.254145
GGCATAG216200.015.95181235
GAGCTTA219300.015.80273344
ATAGGGG229050.015.09652538
TGGCATA240800.014.44363734
CATAGGG243650.014.17324837
GTTGTCG27600.014.02022426
GGGAGGT358800.013.6894033
GTCACTC327250.013.6787248
ACCCGGC96000.013.5014765
GGTCACT331150.013.4085197
GCATAGG258550.013.34763736
ACTCTCC333700.013.32665711
GGAGGTC361050.012.7633634
GAGGTCA379150.012.178575
AGGTCAC376850.012.0980656
TCACCCG111350.012.0835393
ATAGCTC101200.011.96027739
TAGCGTA11900.011.72120237
TAGGGGA376250.011.67193239
Produced by FastQC (version 0.11.5)