FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52024811
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT913990.17568348302120693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT165850.027.2661276
GACCGCG169850.026.6637445
AGACCGC172850.026.2530294
CCGCGTT173650.026.0154787
CAGACCG178850.025.560993
CGCGTTC178950.025.2575458
GAACGAT185050.024.18574745
AATACGG205250.021.88172732
TACGGAG207850.021.55104334
ATACGGA209900.021.38343833
TCAGACC223450.020.8420282
AGAACGA214300.020.83337644
TTCGACG29600.020.5977826
CAATACG223800.020.13848531
AAGAACG232600.019.3007743
GCGTTCT240800.018.9195799
ACGGAGA241800.018.618635
CCCAATA257550.017.73568329
CTCAGAC300000.017.5229261
CGGAGAG258450.017.3060136
Produced by FastQC (version 0.11.5)