FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46015222
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT854150.18562335741855163No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG832850.18099445440032866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA191250.023.12446823
ACGCTAC192350.023.0069724
ACCGCGT180200.022.9187556
GACCGCG180850.022.8165635
AGACCGC187950.022.0743544
CGCTACC203650.021.62163725
CCGCGTT191550.021.5721787
CGCGTTC191850.021.4797748
AAAACGC207150.021.47883421
GAACGAT195150.020.85144245
CAGACCG204600.020.4649243
AAACGCT219350.020.2539722
GAAAACG220250.020.23195820
TCACGCT233350.019.9442988
ACGCTCA253550.018.92314510
TTCGACG18000.018.7452776
GCCGGCT241150.018.5920811
GTTTTCG245350.018.50125545
TCGGCGC67150.018.4239545
CGTGGTT248700.018.33421941
Produced by FastQC (version 0.11.5)