FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46015222
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG489190.1063104726518542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG159400.015.45790645
GGCATAG166550.014.721168535
ATAGGGG182100.013.79710638
TGGCATA186000.013.27849634
GGGAGGT271400.013.1512173
GTTGTCG20400.013.12335826
GAGCTTA191200.012.94394444
GGAGGTC262600.012.7965844
CATAGGG199800.012.39428437
GCATAGG208200.011.84043136
TAGCGTA9550.011.5404437
CGTCGAT27800.011.41741812
ACTCTCC302750.011.15320611
GCGTCGA28850.011.07988311
TGCGTCG29800.010.87774410
ACCCGGC71850.010.5527915
TGGGAGG257850.010.327312
TCACCCG78950.010.3122943
GTCACTC336850.010.102548
TAGGGGA324450.010.0942639
Produced by FastQC (version 0.11.5)