FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48568612
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA521090.10728945682038432No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG503520.10367189410312981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG167100.015.04206345
ATAGGGG173450.014.47327238
GGCATAG172550.014.43079235
GTCACTC257500.014.0190598
GGTCACT267700.013.3328017
GAGCTTA190450.013.26771944
AGGTCAC283900.013.0397126
GTTGTCG25700.012.77955826
GGGAGGT301450.012.7107013
ACTCTCC288050.012.61927911
CATAGGG201050.012.53052437
GAGGTCA309450.012.114725
TGGCATA207250.012.01492134
GGAGGTC318500.011.8478494
GCATAGG212550.011.81073136
TCACTCT321500.011.6414389
TAGCGTA7600.011.5426737
CACTCTC333900.011.00097910
TCTATAG331950.010.3634633
AGGGATC147700.010.3282972
Produced by FastQC (version 0.11.5)