FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48472925
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT909010.18752943008906517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC198400.023.2683734
ACCGCGT203500.022.6520716
GACCGCG208100.022.0756785
CAGACCG210800.022.0703353
CCGCGTT220750.020.8410637
CGCGTTC229550.020.0617038
TTCGACG15550.019.6760946
TCAGACC251050.019.0696682
CTCAGAC304900.017.3915541
GCGTTCT270100.017.1663689
AACGCTA168450.015.69457923
ACGCTAC170050.015.57385224
CGCTACC174150.015.20821725
CTACACG48450.014.90597645
GAACGAT305050.014.8390545
CGTTCTC330300.014.48713710
CGTGGTT190550.014.07445941
AAAACGC190400.014.01418521
TCGACGA23200.013.9637397
GTCCGTT32900.013.8131161
Produced by FastQC (version 0.11.5)