FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48472925
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG140450.014.30309845
ATAGGGG148850.013.67642438
GGCATAG145350.013.59112835
GTTGTCG27650.012.69933426
GAGCTTA160200.012.62423244
CATAGGG164150.012.18481737
TGGCATA168600.011.90397834
GCATAGG170100.011.81173536
GGGAGGT250950.011.54198553
GGAGGTC242350.011.2475584
CCGTACG14300.010.8341684
CGTACGC14700.010.6920835
ACTCTCC267850.010.00245611
TAGGGGA274150.09.56380139
ACCCGGC59950.09.4008295
ATATAGC44800.09.3726086
TGTCGTT40200.08.98595728
AGGGATC137450.08.9682532
TTACGCG12600.08.94882235
TTAGCCC51800.08.9293072
Produced by FastQC (version 0.11.5)