FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53691710
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG748150.13934180900552431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA212900.019.05360623
ACGCTAC213700.019.01384424
CGCTACC219400.018.39706825
TCACGCT237050.017.766428
GCCGGCT236850.017.373241
AAAACGC240250.016.94064321
AGACCGC65650.016.2446544
AAACGCT253250.016.08898222
CGTGGTT253900.016.08792941
GTTTTCG250450.016.03109745
GAAAACG256150.015.88908720
TTCACGC270650.015.8683587
CCCGTAT33350.015.8617111
CCGGCTT285850.015.6486232
TTCGGCG90800.015.61501844
TCGTGGT266100.015.43200940
ACGCTCA279600.015.3845510
CACGCTC289350.014.6484439
GTCCGTT39650.014.5904251
GTTAGTT136750.014.48248744
Produced by FastQC (version 0.11.5)