FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53691710
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN684940.12756904185022233No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG572650.10665519872620932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG195000.015.62542545
GGCATAG201100.015.11631935
ATAGGGG213200.014.60708138
GGGAGGT331550.014.6070583
GTTGTCG26050.014.51203426
GTCACTC310000.014.2575178
GGTCACT317400.013.8891727
GGAGGTC327950.013.8548864
GAGCTTA224350.013.68148544
ACTCTCC328700.013.4265311
TGGCATA230700.013.39166734
AGGTCAC347550.013.0530546
CATAGGG237950.012.84156837
GAGGTCA362300.012.7464355
GCATAGG254300.012.09600536
TCACTCT378650.011.898439
GCGTAGG119150.011.46342736
CGTAGGG119000.011.4585537
CACTCTC388050.011.44837710
TAGGGGA393000.011.36541639
Produced by FastQC (version 0.11.5)