FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48202392
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1176690.24411444145759406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT263600.023.22781845
AGACCGC276750.022.055994
ACCGCGT276400.021.8641196
TTCGACG23750.021.7885446
GACCGCG280100.021.6072985
CCGCGTT287700.020.9739697
CAGACCG294050.020.8730453
CGCGTTC291700.020.686258
TCAGACC327750.019.270582
AGAACGA318700.019.24726544
TCGACGA29350.018.1677847
ATACGGA334800.018.10564233
AATACGG336500.017.9807432
GCGTTCT338300.017.963139
TACGGAG335950.017.88961834
CTCAGAC409500.017.2608181
CAATACG362700.016.78729431
AAGAACG370400.016.5301943
ACGGAGA365900.016.46219635
CCCAATA381800.016.27760329
Produced by FastQC (version 0.11.5)