FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53575684
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA577780.10784370013829409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG157800.012.08975738
GTTGTCG37850.011.88677726
AGCTTAG167750.011.60010845
GGCATAG166300.011.56635635
GGAGGTC236300.010.7789134
GGGAGGT242700.010.7640583
CGTAACG16450.010.53025742
GAGCTTA192550.010.33959244
CATAGGG187750.010.26903537
GTCACTC243850.010.25268
CGTGGCG106450.010.0916131
TGGCATA197600.010.03026634
ACTCTCC255350.09.97421411
GGTCACT248450.09.8636227
GCATAGG195050.09.86148836
CGACCCG108350.09.6995346
AGGTCAC271900.09.5181286
GGCGACC112400.09.5170734
GAGGTCA270350.09.504575
TGTCGTT51250.09.481256528
Produced by FastQC (version 0.11.5)