FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48912676
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1170860.2393776206396886No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT516820.10566177160292764No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG513100.10490123255574894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG89600.033.592376
TCGACGA98250.031.0477
GTTCGAC97000.031.0296525
CGACGAG99400.029.895998
CGGTATA136300.027.84955820
AGTTCGA110450.027.3944954
CAGTTCG122350.025.3184783
ACGAGGA141550.024.69662910
GACGAGG143250.024.2151019
CGAGGAT138150.024.0017611
ATGACGG157400.023.19677716
GTATAGA182950.021.95501722
GCCGGCT278000.021.8681771
CCGGCTT317550.021.6165142
GACCGCG82300.020.8280055
ATAGAGT192800.020.81000924
TATAGAG199200.020.53678723
ACCGCGT84250.020.3993326
TAGAGTT199600.020.1574825
GACGGTA191050.020.0297918
Produced by FastQC (version 0.11.5)