FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48912676
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG725830.14839302597142712No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA707620.14467006466789917No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG513320.1049462106714423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG53500.018.58670226
ATATAGC60450.017.686
AACTCTA167050.017.02537345
GGAGGTC304950.016.313094
GGGAGGT318750.016.009193
TGTCGTT63250.015.68639128
GCTAAGT64150.015.50100814
TATAGCT72450.014.5964897
GTCGTTC68900.014.26968229
GAGGTCA355550.014.2257385
AGCTTAG262250.014.19967945
GGCATAG269500.014.0114635
TCGTTCC70700.014.00249130
ATAGGGG279600.013.81146138
GGTCACT354650.013.7926767
GTCACTC354300.013.7806598
CGTTCCC74400.013.51834531
ACTCTCC364300.013.32203611
CATGCAT112000.013.1584479
TATATAC89650.013.126247
Produced by FastQC (version 0.11.5)