FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43999557
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT981110.22298179047575412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC189600.026.7954944
GAACGAT196000.025.57720245
ACCGCGT199300.025.4913486
CAGACCG205800.024.9704783
GACCGCG205050.024.8204125
CCGCGTT208150.024.3317747
CGCGTTC213350.023.7809168
AGAACGA202500.023.31172444
TCAGACC236150.022.0566832
ATACGGA229850.022.02558733
GCGTTCT232200.022.0247969
TACGGAG233350.021.67594134
AATACGG234950.021.54748332
TTCGGCG82200.020.3928744
ACGGAGA252800.019.9993335
AAGAACG237550.019.8625243
CTCAGAC301350.019.7553021
CAATACG256350.019.74870931
CCCAATA270450.018.87659629
CGGAGAG273350.018.52044536
Produced by FastQC (version 0.11.5)