FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43999557
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG668200.15186516536973316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG175950.018.82137145
GGCATAG185950.017.64007635
GTTGTCG27100.016.52030226
GAGCTTA204000.016.3660344
ATAGGGG205450.016.10813138
CATAGGG207150.016.04090137
TGGCATA206550.015.96828434
GCATAGG218300.015.19084436
AGCGCTC77500.015.01153859
ACCCGGC75800.014.8159475
TCACCCG80000.014.4043083
GGGAGGT317000.014.2779293
GGAGGTC308150.013.9647364
ACGGACA81400.013.76496618
TAGCGTA6950.013.59557937
ACTCTCC317950.013.24084911
ATAGCTC78350.012.72041739
TGGGAGG279550.012.5520692
TAGGGGA330000.012.55094739
ATAGCGT8250.012.54417336
Produced by FastQC (version 0.11.5)