FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42844985
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG537450.12544058540340253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA141600.019.46513723
ACGCTAC143150.019.27150724
CGCTACC147500.018.65894125
CTACACG52850.017.6232645
AAAACGC158000.017.5715621
GAAAACG163950.017.05680720
GTCCGTT32000.017.0292841
CTCGGTT82150.016.5561891
TCACGCT178100.016.0923968
AAACGCT177000.015.69893622
ACGCTCA185200.015.49983110
GCCGGCT190800.014.6225791
CCGTACG57300.014.6049984
TTCACGC202700.014.472357
CGATTAG21400.014.4019064
CTCCGTT69400.014.3414751
GTTTTCG194650.014.27392845
CCGGCTT221550.014.1446622
GTCCGCT41650.014.0028291
ACGGTAC66050.013.99909445
Produced by FastQC (version 0.11.5)