FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53844427
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30850.020.273726
ACCGCGT100100.017.1937716
TCGACGA38600.016.7277857
AACGCTA116500.016.64873323
AGACCGC104500.016.5791324
ACGCTAC117050.016.57064224
GACCGCG104800.016.4872325
CCGCGTT106800.016.0730067
CGGTATA51000.015.92562820
CGCTACC123050.015.68956125
GTCCGTT39400.015.607031
CGACGAG39700.015.5275818
CGCGTTC111900.015.2801398
CAGACCG117950.015.0319613
CCCGTAT32250.014.94644451
CGTGCGA23300.014.58099138
TACGGTA71000.013.94285944
AAAACGC141950.013.83725921
ACGGTAC71650.013.78493245
GAACGAT121300.013.651278545
Produced by FastQC (version 0.11.5)