FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41690382
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC19050.012.04926614
ATAGGGG64000.011.70482438
CCGCGTT19750.011.62221713
GCGTTCG19050.011.22235515
CATAGGG65400.011.14467637
GGCATAG66550.011.01979235
CGTTCGC20750.010.73685816
ACCCGGC46950.010.1605115
GCATAGG71600.010.05398436
AGCTTAG76600.09.86723745
GTTGTCG16950.09.42500426
GAGCTTA88800.08.86626344
GGGAGGT130200.08.4683813
TAGGGGA112500.08.15846339
TCACCCG64600.08.150773
CTTGACG92450.08.126904542
TCAGACG55200.08.11156752
TGGCATA94100.08.08054734
CACCGAA104400.07.97234945
ACGGACA70250.07.94476618
Produced by FastQC (version 0.11.5)