FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45565838
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG999150.219276116462513No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG527240.11570949271250099No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC479770.10529160025543698No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT458000.10051389815326121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC100350.023.8540654
ACCGCGT110200.021.6982656
GACCGCG112100.021.4332775
CAGACCG112750.021.2717693
TTCGACG18850.021.1220116
GAAAACG269900.020.41947720
AACGCTA283750.020.40835423
ACGCTAC285700.020.27032724
CGCTACC290750.019.87267725
AAAACGC301450.019.29049521
AAACGCT313050.018.61970722
GTTTTCG314350.018.39773445
TCACGCT364000.018.1808098
GCCGGCT341650.017.674111
TCGGCGC137550.017.59654445
TTCACGC392600.017.1944437
ACGCTCA394850.017.1420210
TTCGGCG142800.016.99685344
CCGTACG143850.016.8595924
TCAGACC145600.016.8433342
Produced by FastQC (version 0.11.5)