FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45565838
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG846580.1857926984685325No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA816630.17921979180982034No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG704540.15462022228143812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG215000.019.37774845
GGCATAG237850.017.62727735
ATAGGGG247600.016.97782338
GAGCTTA250400.016.69122744
TGGCATA258450.016.32538434
GGGAGGT361850.015.9035563
GGAGGTC355550.015.8652074
GTTGTCG25250.015.50580626
ACTCTCC368150.015.08950611
CATAGGG282050.014.98451637
GCATAGG298800.014.18968636
GTCACTC402500.013.9462378
GGTCACT400850.013.8394837
TGGGAGG331600.013.4398282
TAGCGTA8600.013.33870837
TCAGACG104150.013.1547242
TGTCGTT29450.012.9121728
TCTCCCC444400.012.51625513
CACTCTC451600.012.40582510
GCTCTGT454800.012.31925122
Produced by FastQC (version 0.11.5)