FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50050939
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1101230.22002184614358586No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT603370.12055118486388437No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT567610.11340646376284769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA280900.021.0347323
ACGCTAC282150.020.93375624
CGCTACC289000.020.40647325
TCACGCT304150.019.971878
TTCGACG28500.019.7350376
TTCACGC310350.019.7106197
AAAACGC300850.019.6984321
CCGGCTT326350.019.6773092
GCCGGCT303100.019.6635931
AGACCGC134900.019.6317864
ACCGCGT135950.019.444716
GAAAACG308600.019.2179120
GACCGCG138300.019.0818825
AAACGCT314200.018.91907122
GTTTTCG319300.018.5388245
TCGGCGC116850.018.33025245
CGTGGTT325600.018.08328441
CAGACCG148750.018.0156573
TTCGGCG118650.018.01427844
GTTAGTT157100.017.90172444
Produced by FastQC (version 0.11.5)