FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50050939
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG849580.16974306915600526No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA521000.10409395116443271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT368150.015.3873823
AGCTTAG270200.015.13543945
GGAGGTC384750.014.6464974
GGCATAG292700.013.89569635
ATAGGGG297550.013.82315238
ACTCTCC409500.013.57947911
GAGCTTA313300.013.25142144
GTCACTC428150.013.1248828
GGTCACT419300.013.1232697
TGGCATA313950.013.01967734
TGGGAGG330500.013.0037812
CACTCTC478900.011.62712510
CATAGGG356700.011.57590937
CTGGGAG413850.011.5429081
GAGGTCA503550.011.3266915
TCTCCCC497350.011.26081213
TATCCTA101100.011.2563035
AGGTCAC507150.011.2097666
GCTCTGT498950.011.15463422
TGCCCGT166050.011.13330544
Produced by FastQC (version 0.11.5)