FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46669437
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG768370.16464094049388256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG263150.015.11174145
GGGAGGT384050.014.8874883
GGAGGTC367450.014.8498874
GTCACTC388300.013.7644528
GGCATAG289100.013.760509535
GGTCACT390950.013.6884217
ACTCTCC392900.013.51686311
GTTGTCG27750.013.45780626
ATAGGGG302300.013.28752838
GAGGTCA421050.013.2068295
GAGCTTA307400.013.0059244
AGGTCAC424900.012.9706856
TGGCATA316500.012.68298534
TGGGAGG340000.012.4815422
TCACTCT435500.012.4483389
CACTCTC436750.012.144773510
CATAGGG344800.011.60402337
ATATAGC39000.011.30973156
CTGGGAG418650.011.1704141
ACCCGGC79900.010.9844245
Produced by FastQC (version 0.11.5)