FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50795733
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA649050.1277764807528223No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG574480.11309611380152738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG177100.013.97408145
ACCCGGC96900.013.40333755
CGGACAC101950.013.08927113
GGCATAG188200.013.07727635
GTCACTC259450.012.9849958
ACGGACA100250.012.90368718
GGTCACT272850.012.2655537
GAGCTTA209950.011.99170444
AGGTCAC297750.011.8226576
GGGAGGT315900.011.8212573
ATAGCTC104450.011.76088939
CGGACAG110100.011.72904419
TCAGACG160200.011.718922
TGGCATA211900.011.71048534
GAGGTCA298750.011.7010415
ATAGGGG221900.011.62911638
ACTCTCC298750.011.58543211
TCACCCG125450.011.2682353
GGAGGTC315950.011.2067194
CCGGACA121150.011.18191912
Produced by FastQC (version 0.11.5)