FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48590035
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1158880.23850157753539386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT264050.024.71860145
AGACCGC295150.022.5980264
ACCGCGT294400.022.5165966
GACCGCG301150.022.0059345
CCGCGTT305500.021.72787
CAGACCG311450.021.5597723
CGCGTTC311650.021.2842038
AGAACGA310000.021.05482144
TCAGACC349600.019.6188772
AATACGG335300.019.3296432
TACGGAG337350.019.15894934
ATACGGA343450.018.87752233
GCGTTCT360200.018.5402579
AAGAACG359050.018.23327443
CAATACG359800.018.16973331
ACGGAGA365550.017.71481935
CTCAGAC437300.017.2018951
CGGAGAG380300.016.8973736
CCCAATA399050.016.60960429
AACGCTA153000.016.570923
Produced by FastQC (version 0.11.5)