FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48590035
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG152850.012.83843345
GGCATAG159350.012.15591935
GTTGTCG31100.011.93611426
ATAGGGG171550.011.56750438
GGGAGGT268700.011.412813
GAGCTTA182300.010.8631844
TGGCATA181050.010.74851734
GGAGGTC269200.010.7397094
ACTCTCC276300.010.09095411
CATAGGG195950.010.02376237
GTCACTC286250.09.8179078
GGTCACT283400.09.7964377
ACCCGGC73350.09.7235125
GCATAGG204750.09.57079536
TCAGACG94400.09.4858252
TGTCGTT42050.09.14875428
TTACGCG18350.09.07261435
ATAGCTC75400.08.83314139
ATATAGC48350.08.654996
GCTAAGT46550.08.60482614
Produced by FastQC (version 0.11.5)