FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50114105
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG941350.1878413273069528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG255300.018.76752545
GTTGTCG43800.017.21576126
GGCATAG287700.016.5485735
ATAGGGG300600.016.09507838
GAGCTTA302250.015.96420344
GTCACTC399900.015.7727828
GGTCACT406800.015.5987467
TGGCATA319150.015.07297634
GGGAGGT455900.014.912563
GGAGGTC437700.014.9017944
CATAGGG325950.014.72274637
ACTCTCC428200.014.64719211
ATATAGC56450.014.5180796
AGGTCAC445850.014.4736566
GAGGTCA459000.014.2347035
GCATAGG346500.013.85621236
TAGCGTA9500.013.76805537
TGTCGTT56300.013.755428
TCACTCT467050.013.67332659
TATAGCT62050.013.5702527
Produced by FastQC (version 0.11.5)