FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52420203
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG712960.13600863010774683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG224400.016.01286145
GGCATAG247400.014.44806235
ATAGGGG254450.014.26915738
GGGAGGT364000.014.05219653
GTCACTC342550.013.90096958
GGAGGTC364000.013.5273734
GGTCACT356250.013.4673057
GAGCTTA269250.013.43021644
GTTGTCG32950.013.23573626
AGGTCAC379250.013.0236026
GAGGTCA387250.012.7437615
TGGCATA283000.012.73864234
ACTCTCC377950.012.65904611
CATAGGG292200.012.45346637
GCATAGG301000.012.02946336
TCACTCT423550.011.3804629
CACTCTC418150.011.29040910
TGGGAGG355450.010.86255552
TAGGGGA444500.010.72281839
AGGGGAG472000.010.23589240
Produced by FastQC (version 0.11.5)