FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52997515
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG845360.1595093656749755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG233650.018.46130445
GGCATAG270550.015.77954435
ATAGGGG276700.015.613173538
GAGCTTA283300.015.29861344
GTCACTC382400.014.8870578
GGGAGGT413800.014.473353
GGTCACT391800.014.4449727
TGGCATA299500.014.27692534
GGAGGTC414600.014.0600064
CATAGGG310600.013.83819437
ACTCTCC414350.013.74613211
GAGGTCA432050.013.6096695
AGGTCAC439400.013.4200666
GCATAGG326500.013.21254436
TGGGAGG376550.012.1058292
TAGGGGA475750.011.97956739
TCACTCT484950.011.9423059
TAGCGTA9850.011.879951537
CACTCTC492150.011.53426710
AGGGATC154600.011.4827332
Produced by FastQC (version 0.11.5)