FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48633157
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT727840.14965921295218404No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG594780.12229927824755445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC180150.023.5174314
ACCGCGT181300.023.2707886
CAGACCG194450.021.9583383
CCGCGTT194900.021.6243027
CGCGTTC195450.021.5747538
GACCGCG196600.021.3923745
GAACGAT209000.020.12564545
TCAGACC228050.019.33332
GCGTTCT231100.018.3421659
AGAACGA237800.017.81828544
AATACGG238150.017.64200632
ATACGGA238350.017.62720333
TACGGAG236650.017.52047334
AACGCTA223100.017.049423
ACGCTAC225200.016.88069324
CGCTACC228100.016.6273425
CTCAGAC309800.016.410751
CAATACG261950.016.2077631
AAAACGC238150.016.1018121
ACGGAGA263200.015.94614635
Produced by FastQC (version 0.11.5)