FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48633157
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG184850.016.13587645
GGCATAG191600.015.66193235
GGGAGGT288750.014.9663273
TGGCATA208900.014.35294734
ATAGGGG212950.014.33202538
GTTGTCG20700.014.05174226
GAGCTTA214200.014.03958744
GGAGGTC290850.013.864444
CATAGGG222350.013.54636737
GCATAGG237900.012.77293236
TGGGAGG248500.012.4699242
TCAGACG66400.012.4354272
TAGCGTA10550.011.79059837
ACTCTCC331000.011.75432811
ACCCGGC75350.011.5812865
CGTCAGT98050.011.4021131
AGGGATC135200.011.2858072
TCTCCCC350650.011.18416813
CTGGGAG329900.010.9851611
TAGGGGA359300.010.88000139
Produced by FastQC (version 0.11.5)