FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51659156
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG49800.015.44890526
ATATAGC61850.014.9568686
TATAGCT67800.013.9423727
GGGAGGT276050.013.5306133
GCTAAGT60850.013.49604114
ATAGGGG207550.012.88808138
GGAGGTC286450.012.8821694
GGTCACT289750.012.4671937
GGCATAG211800.012.45891335
GAGGTCA302750.012.4042335
AGCTTAG219850.012.30984845
GTCACTC301600.012.096268
AGGTCAC314300.011.9594336
TGTCGTT67800.011.54642528
ACTCTCC321600.011.32850511
GAGCTTA240700.011.1314444
CAATATA93150.011.02150254
TGGCATA244550.010.91920934
TTGTCGT80450.010.70954927
CTAAGTG80650.010.43357915
Produced by FastQC (version 0.11.5)