FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40270226
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA556310.13814424582568768No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG426590.10593186142039532No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGTC403690.10024527798776198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37900.025.3487286
TCGACGA43250.022.6295577
GTTCGCT52850.021.8247781
CGACGAG44350.021.5102338
GCACTAC108550.020.41640725
GTTCGAC47200.019.8774915
GTCCGTT33000.018.9782311
CGGTATA64750.018.59025420
CTCGGTT82750.018.4580821
CCCAGAT178750.018.249351
CCCGGAT83450.017.3313981
GTTCCTA87000.017.22367115
GCGTATG85600.017.21611238
AGCACTA135850.016.49581124
CCCGTAT27050.016.40681
CGGTTTT83850.016.3947813
GTCGGAT36650.016.3505551
CCGGCTT162900.016.297782
CGTATGC91500.016.25353639
GCGGACT95950.016.1797432
Produced by FastQC (version 0.11.5)