FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53026061
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG708040.13352679543743595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG55550.018.4295125
GCCGGCT211450.018.3069341
ACCGCGT56550.018.101776
AGACCGC58850.017.5872464
CCGGCTT265950.017.2170812
TTCGACG15950.016.6442246
TCGTTAC27950.016.50163744
TCACGCT268550.015.975998
TTCACGC272050.015.911047
CCCGTAT34400.015.8417941
ACGCTCA279550.015.44392710
TCGTATG39600.015.11241945
AACGCTA279300.014.98447723
GCACTAC63250.014.97702925
ACGCTAC281350.014.93943124
CCCGGAT94150.014.8292371
CACGCTC291450.014.7670329
CCGCGTT69250.014.7170477
GTCCGTT38550.014.6621211
CGCTACC286150.014.65737625
Produced by FastQC (version 0.11.5)