FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52964105
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTC95350.013.40430639
ACCCGGC106900.012.9210415
AGGGATC142050.012.6394062
TAGCGTA12650.012.45137337
ACGGACA107300.012.369884518
AACTAAC174850.012.28987437
TAGCTCT107100.012.24821640
CGGACAC114650.011.91042213
CGGACAG113500.011.63499619
TCACCCG128600.011.6161943
CACCCGG130050.011.434234
TGATAGC121200.011.34353337
AGCTTAG182800.011.20607945
GTTAGTT63000.011.0774251
TAGGCTA87150.010.73828210
CTAACCA199800.010.65399239
CCGGACA137100.010.59995212
ACACGGA130050.010.22326616
TATCCTA98550.010.1580475
ATAGCGT15950.010.15742436
Produced by FastQC (version 0.11.5)