FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45688627
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1598730.34991859133784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT277450.028.09180845
AGACCGC315500.024.803984
ACCGCGT313950.024.7477256
GACCGCG314950.024.654895
CCGCGTT326650.023.7855977
CAGACCG330500.023.767153
AGAACGA332950.023.50742544
CGCGTTC335000.023.138998
AATACGG350850.022.23198732
ATACGGA351850.022.12986633
TACGGAG353400.022.02699534
TCAGACC366800.021.7217622
CAATACG372900.020.95444331
AAGAACG375450.020.84129743
GCGTTCT376400.020.701819
ACGGAGA381000.020.46181135
TTCGACG22400.020.084246
CTCAGAC436300.019.6762031
CGGAGAG399500.019.46862436
CCCAATA411700.019.22103529
Produced by FastQC (version 0.11.5)