FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56891206
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1131850.19894990448963237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT333550.018.0945241
ACGCTAC350650.017.74747324
AACGCTA352900.017.6783723
CGCTACC360650.017.24915325
TCACGCT377000.017.0116048
TTCACGC388550.016.51757
CCGGCTT405150.016.5038572
AAAACGC381950.016.37970521
ACGCTCA409000.016.3571310
CGTGGTT368200.016.1851641
GAAAACG392900.015.9345620
AGACCGC100300.015.8192854
AAACGCT398850.015.73196422
CTACACG107050.015.71790245
CGGCTTC446150.015.6833373
GTTTTCG397750.015.55273745
GTCCGTT44050.015.5359921
GTTCCTA84800.015.51849115
TCGTGGT383200.015.504642540
CCCGGAT161100.014.9100891
Produced by FastQC (version 0.11.5)