FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56891206
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA742000.13042437525405948No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG610450.10730129363051295No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG596440.10483869862066204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG185200.015.77485345
GGCATAG210000.013.80885135
GAGCTTA219100.013.37242444
ATAGGGG224900.013.30459938
GGGAGGT326800.013.1304163
GGTCACT333050.012.1558747
CATAGGG242400.012.12111437
TGGCATA242550.012.11339534
ACTCTCC341200.011.9913911
GGAGGTC342350.011.9032084
GTCACTC353650.011.6071388
GCATAGG252400.011.59612936
GTTGTCG27650.011.47217226
ACCCGGC86850.011.2709915
TCACCCG96050.011.1979973
GAGGTCA392050.010.7450155
AGGGATC178650.010.6799832
TAGCGTA10200.010.58700337
CACTCTC386250.010.51703710
AGGTCAC399550.010.4873956
Produced by FastQC (version 0.11.5)