FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47294583
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1160650.24540865494046116No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG540760.11433867595364991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC251700.026.0270564
GACCGCG255900.025.6343425
ACCGCGT263150.024.9452726
CAGACCG267250.024.655773
TTCGACG26350.023.4778546
TCAGACC302350.022.0919282
CCGCGTT308750.021.2391877
CGCGTTC325100.020.1641888
TCGACGA31350.020.020417
CTCAGAC370100.019.575981
GCGTTCT367200.017.925899
CGGTATA44950.017.8670620
CTACACG45700.017.08206245
CGACGAG36100.016.887698
TTCGGCG82350.016.63825444
TCGGCGC85500.016.1821445
AACGCTA150050.015.42860223
ACGCTAC150550.015.34846324
CGTTCTC437600.015.24250810
CCGTACG92900.015.1349564
Produced by FastQC (version 0.11.5)