FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47989996
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG650360.13551991127484153No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA616500.1284642740957928No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG600690.12516983748029487No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG505700.1053761288081791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32400.014.65192526
GGGAGGT360700.013.0645153
GTCACTC333000.012.9537738
AGGTCAC348300.012.9206136
GGTCACT341300.012.750547
ATATAGC43650.012.6295666
GAGGTCA366500.012.5873815
GGAGGTC362550.012.5322064
AGCTTAG264250.012.11611745
ACTCTCC375400.011.60970411
ACCCGGC74100.011.570775
GGCATAG282550.011.42683935
TCACTCT390350.011.1486089
TGTCGTT43400.011.09165128
ATAGGGG299050.011.03675238
TGGGAGG319850.011.0109752
CACTCTC405650.010.5614810
TGGCATA309900.010.51263234
GAGCTTA309950.010.42399244
TATCCTA121800.010.4019945
Produced by FastQC (version 0.11.5)