FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43954455
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA95150.018.84795623
GTCCGTT43500.018.5894181
ACGCTAC97950.018.33299424
ACGTTCG17150.017.8407026
CGCTACC101050.017.77096225
AAAACGC115500.015.72096121
TCACGCT121150.015.61743458
GCCGGTT73550.015.3125651
GTCCGAT33250.015.3100791
GCGGACT72600.015.1223872
ACGCTCA132000.014.87921210
GAAAACG122300.014.82758520
CCCGTAT31750.014.8273391
ACGGTAC59200.014.55506645
CGGACTT83150.014.5023933
GCGTATG74000.014.19784238
GCACTAC48950.014.15931925
TACGGTA60300.014.06569444
CCTTACG61800.013.94271841
CGATTAG27100.013.8638654
Produced by FastQC (version 0.11.5)