FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49628192
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG797670.1607292081081656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG202600.018.92954345
GGCATAG232850.016.34109135
GAGCTTA246050.015.60517744
ATAGGGG251650.015.35407938
GTTGTCG27950.015.29315426
TGGCATA255750.014.97462234
CATAGGG263200.014.69661137
ACTCTCC377500.014.29178211
GTCACTC386850.013.9344158
GGGAGGT419800.013.9239853
GGTCACT385600.013.909817
TAGCGTA11700.013.8475837
GCATAGG280000.013.79839536
GGAGGTC398650.013.6468174
ACCCGGC99450.013.5060555
GAGGTCA420500.013.2103425
AGGTCAC423800.012.99576956
TGTCGTT34700.012.642426528
TCACCCG111800.012.6381853
CACTCTC433750.012.39668510
Produced by FastQC (version 0.11.5)