FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46186010
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT908440.19669159557190585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30100.027.949376
GACCGCG175250.026.819685
ACCGCGT176100.026.773116
AGACCGC179550.026.603444
CCGCGTT190550.024.7188667
CAGACCG196450.024.5095273
CGCGTTC194800.024.2141068
TCGACGA36350.023.4528487
GAACGAT211450.022.63720745
CGGTATA51400.022.2748620
TCAGACC231150.021.6673762
CGACGAG39800.020.7415838
GCGTTCT236450.020.0535019
TACGGAG235900.019.90003434
ATACGGA242800.019.60342633
AATACGG243100.019.55178832
CTCAGAC302400.019.3550511
AGAACGA249000.019.2415144
CAATACG262950.018.358431
ACGGAGA265350.017.81311635
Produced by FastQC (version 0.11.5)